Biochemical Characterization and Antimicrobial Resistance of Bacterial Isolates from Post-Surgical Wounds in Jinnah Hospital Lahore, Pakistan

Abstract

Objective: The current study was aimed to determine the antimicrobial sensitivity pattern of clinical isolates from post-surgical wounds.

Material and Methods: A retrospective study was carried out in Jinnah Hospital Lahore, Pakistan. Total of 150 post-surgical hospitalized subjects were enrolled. Specimen were collected from surgical site (wound). Cultured were done on Blood and MacConkey agar. Bacterial isolates were identified using morphological and biochemical characteristics. Antimicrobial susceptibility were assessed according to CLSI guidelines.

Result: Among 150 specimen 78 (25%) were wound swabs and 113 (75%) were pus specimen. Among cultured specimens, 115 (76.7%) were clinically significant. S.aureus, Pseudomonas, Acinetobacter and E. coli were isolated from these specimens. Staph aureus show (83.3%) resistant against Erythromycin, (50%) to penicillin, (50%) against Ciprofloxacin,(17%) resistant for Gentamicin, (9%) resistant for Methicillin. Acinetobacter species show resistant against Amikacin (33.3%), Ampicillin (44.4%), and Ciprofloxacin (77.8%) Erythromycin (33.3%), Tazobactum (42.9%), Imipenum (66.7%), Meropenum (55.6%), Augmentin (72.6%), Lenzolid (77.8%) resistant show. E coli were found resistant to Amikacin, Ampicilin, and Ciprofloxacin.

Conclusion: Bacterial infection is a significant problem in post-surgical patients admitted in hospital. Most prevalent microorganism are Staph-aureus, Pseudomonas, E.coli, and Acinetobacter. Multidrug resistance pose a life threatening risk to post-surgical patients.