Correlation of blood Complete Performed by Hematology Analyzer with manual methods at District Bannu Khyber Pakhtunkhwa, Pakistan

Abstract

Objective: To measure the correlation between the results of Blood Complete performed by automated Hematology Analyzer Sysmex XP-100 with the manual methods at District Bannu, Khyber Pakhtunkhwa Pakistan.

Methods: This Comparative Cross-sectional descriptive study was conducted in Samad Clinical Laboratory, District Bannu Khyber Pakhtunkhwa, Pakistan, from October 2020 to October 2021. The Selection of 50 patients was made from all those who came to Samad clinical Laboratory for Blood complete determination from District Bannu. Using Standard Operating Procedure for Phlebotomy, 3ml of venous blood sample was obtained into tri-potassium Ethylenediaine tetra-acetic acid (EDTA K3) bottle for the determination of Blood Complete using the automated and the manual methods. Patients of any Age, Sex & Area having Advised Blood Complete tests were included in this study. Those Patients which blood samples were hemolyzed during phlebotomy, during sample processing, improper mixing, poorly prepared and poorly stained slides of blood samples were excluded from this study

Results: Total 50 samples were processed. Statistically significant differences (p < 0.05) were seen between the two methods (Manual & Automated). Similarly, the mean values of platelets, Hemoglobin (HB), Total Leucocyte (TLC), White Blood Cell (WBC), and Red Blood Cells (RBC) showed statistically significant difference (p < 0.001) and correlated positively when both methods were compared.

Conclusion: It can be concluded from the present study that the results obtained from the hematology analyzer correlated well with those by the standard manual method, although the latter method provided additional diagnostic information on the blood pictures.